cu tem grids carbon type-b 400 mesh cu Search Results


carbon type b 400 mesh copper grid  (Ted Pella)
86
Ted Pella carbon type b 400 mesh copper grid
Carbon Type B 400 Mesh Copper Grid, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/pm28985469-148-0-9?v=Ted+Pella
Average 86 stars, based on 1 article reviews
carbon type b 400 mesh copper grid - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
glow discharged carbon type b grids  (Ted Pella)
86
Ted Pella glow discharged carbon type b grids
Glow Discharged Carbon Type B Grids, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/bio_rxiv__64898__2025__12__30__697083-265-14-21?v=Ted+Pella
Average 86 stars, based on 1 article reviews
glow discharged carbon type b grids - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
glass beads  (MACHEREY NAGEL)
94
MACHEREY NAGEL glass beads
Glass Beads, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/pmc06252066-47-73-81?v=MACHEREY+NAGEL
Average 94 stars, based on 1 article reviews
glass beads - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
mesh cu grids  (Ted Pella)
86
Ted Pella mesh cu grids
Mesh Cu Grids, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/pm26502636-59-32-37?v=Ted+Pella
Average 86 stars, based on 1 article reviews
mesh cu grids - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
primary antibodies n93a/49  (NeuroMab)
90
NeuroMab primary antibodies n93a/49
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Primary Antibodies N93a/49, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/pmc12056048-444-8-13?v=NeuroMab
Average 90 stars, based on 1 article reviews
primary antibodies n93a/49 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
cyclin b antibody  (Becton Dickinson)
90
Becton Dickinson cyclin b antibody
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Cyclin B Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/pmc02582542-303-21-24?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
cyclin b antibody - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
mesh carbon type b  (Ted Pella)
86
Ted Pella mesh carbon type b
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Mesh Carbon Type B, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/mui_wilton__2017__development_and_applications_of_opposed_migration_aerosol_classifiers_omacs-4093-11-26?v=Ted+Pella
Average 86 stars, based on 1 article reviews
mesh carbon type b - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
carbon coated copper grids  (Ted Pella)
86
Ted Pella carbon coated copper grids
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Carbon Coated Copper Grids, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/10__1021_slash_acs__chemmater__7b04446____cm7b04446_si_001-75-8-12?v=Ted+Pella
Average 86 stars, based on 1 article reviews
carbon coated copper grids - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
anti-cyclin b mouse antibody  (Becton Dickinson)
90
Becton Dickinson anti-cyclin b mouse antibody
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Anti Cyclin B Mouse Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/10__1074_slash_jbc__m114__581504-62-93-97?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-cyclin b mouse antibody - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
carbon coated grids  (Ted Pella)
86
Ted Pella carbon coated grids
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Carbon Coated Grids, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/bio_rxiv__64898__2026__02__19__706829-183-0-2?v=Ted+Pella
Average 86 stars, based on 1 article reviews
carbon coated grids - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
copper formvar tem grids  (Ted Pella)
86
Ted Pella copper formvar tem grids
A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G <t>GABA</t> B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.
Copper Formvar Tem Grids, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+tem+grids+carbon+type-b+400+mesh+cu/10__1021_slash_acsearthspacechem__1c00237-40-4-13?v=Ted+Pella
Average 86 stars, based on 1 article reviews
copper formvar tem grids - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier

Image Search Results


A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G GABA B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Phylogenetic divergence of GABA B receptor signaling in neocortical networks over adult life

doi: 10.1038/s41467-025-59262-8

Figure Lengend Snippet: A Whole-cell currents from L2/3 (black) and L5 (blue) PCs from 1-, 6–8-, 12–14-month-old rats, and human neocortex following bath-application of baclofen and CGP. Number of cells and baseline current (grey line) indicated. B Peak baclofen current from L2/3 and L5 in 1-month (L2/3: 10 cells/8 rats; L5: 11 cells/5 rats), 6–8-month (L2/3: 22 cells/13 rats; L5: 19 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats (L2/3 vs. L5: p = 7.0 × 10 −6 (1-month), 0.317 (6–8-month), 0.105 (12–14-month). B ’ Peak baclofen current of adult rats and humans (L2/3: 34 cells/19 cases; L5: 12 cells/8 cases). C Baclofen current-density for rat neurons (L2/3 vs. L5: p = 0.0002 (1-month), 0.282 (6–8-month), 0.762 (12–14-month). C ’ Baclofen current density for adult rat and human neurons. D Scatter-plot of baclofen current density for rat L2/3 (black) and L5 (blue) and age (months) showing linear regression (solid lines) with 95% confidence intervals (dashed lines) and R 2 and P -values ( F -test). E Scatter-plot of baclofen current density from human L2/3 (black) and L5 (blue) plotted against age (years) in the same format. F Time-course plots of L2/3 (black) and L5 (blue) PCs from 1-month (L2/3: 8 cells/5 rats; L5: 6 cells/3 rats) and 6–8-month (L2/3: 8 cells/5 rats; L5: 7 cells/5 rats) following bath-application of CGP. G GABA B R-mediated tonic-currents plotted for rat PCs. Data is shown as mean ± SEM ( A , F ), or boxplots (25–75% range) with median indicated and whiskers indicating maximum/minimum ranges ( B , C , G ) or individual data (filled circle) as a scatter-plot ( D , E ). Box-plots are overlain by responses of individual cells (open circles). Statistics shown from LMM ( B , C , G ) or Pearson correlation ( D , E ), with comparisons from 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.

Article Snippet: Sections were incubated in primary antibodies raised against GABA B1 (mouse, 1:400, N93A/49, NeuroMab, UNC Davis, CA, USA) and NeuN (rabbit, 1:500, ABN78, Millipore) diluted in PBS containing 5% NGS, 0.3% TritonX-100 and 0.05% NaN 3 , for 24–72 h at 4 °C.

Techniques:

A Immunofluorescence for GABA B1 (green) and NeuN (magenta) in rat and human neocortex, with layers (dashed lines) and depth (arrow). Scale = 100 μm. B GABA B1 intensity normalised to cortical depth for 1 (black, n = 5), 6–8 (grey, n = 3) and 12–14-month-old (grey, n = 3) rats, with SEM (dotted lines). C GABA B1 intensity for human (magenta, 6 patients) and adult rat (black, 6–14-months). D Schematic of IPSC recordings, with control and CGP from L2/3 (black) and L5 (blue). E IPSC amplitudes in 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 22 cells/13 rats; L5: 17 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. E ’ IPSC amplitude for adult rats and humans (L2/3: 19 cells/13 cases; L5: 8 cells/5 cases). F IPSC 20–80% rise-time in 1-month (L2/3: 18 cells/10 rats; L5: 5 cells/5 rats), 6–8-month (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14-month-old (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats. Rise-time could not be measured for all IPSCs. F ’ 20–80% rise-time in adult rat and humans (L2/3: 14 cells/10 cases; L5: 6 cells/4 cases). G IPSC decay time-constant from 1 (L2/3: 18 cells/10 rats; L5: 6 cells/5 rats), 6–8 (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14 (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats (L5: 1 vs. 12–14 months, p = 0.035, L5: 6–8 vs.12–14 months, p = 0.0013; 12–14-month: L2/3 vs. L5: P = 0.0048). G ’ Decay time-constants of adult rat and human (L2/3: 17 cells/10 cases; L5: 5 cells/5 cases). H IPSC charge-transfer 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 20 cells/13 rats; L5: 15 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. H ’ IPSC charge-transfer for adult rat and humans (L2/3: 22 cells/13 cases; L5: 8 cells/5 cases). Data shown as boxplots (25–75% range) with median indicated and whiskers indicating maxima/minima, showing individual points. Statistics from LMM with 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Phylogenetic divergence of GABA B receptor signaling in neocortical networks over adult life

doi: 10.1038/s41467-025-59262-8

Figure Lengend Snippet: A Immunofluorescence for GABA B1 (green) and NeuN (magenta) in rat and human neocortex, with layers (dashed lines) and depth (arrow). Scale = 100 μm. B GABA B1 intensity normalised to cortical depth for 1 (black, n = 5), 6–8 (grey, n = 3) and 12–14-month-old (grey, n = 3) rats, with SEM (dotted lines). C GABA B1 intensity for human (magenta, 6 patients) and adult rat (black, 6–14-months). D Schematic of IPSC recordings, with control and CGP from L2/3 (black) and L5 (blue). E IPSC amplitudes in 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 22 cells/13 rats; L5: 17 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. E ’ IPSC amplitude for adult rats and humans (L2/3: 19 cells/13 cases; L5: 8 cells/5 cases). F IPSC 20–80% rise-time in 1-month (L2/3: 18 cells/10 rats; L5: 5 cells/5 rats), 6–8-month (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14-month-old (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats. Rise-time could not be measured for all IPSCs. F ’ 20–80% rise-time in adult rat and humans (L2/3: 14 cells/10 cases; L5: 6 cells/4 cases). G IPSC decay time-constant from 1 (L2/3: 18 cells/10 rats; L5: 6 cells/5 rats), 6–8 (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14 (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats (L5: 1 vs. 12–14 months, p = 0.035, L5: 6–8 vs.12–14 months, p = 0.0013; 12–14-month: L2/3 vs. L5: P = 0.0048). G ’ Decay time-constants of adult rat and human (L2/3: 17 cells/10 cases; L5: 5 cells/5 cases). H IPSC charge-transfer 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 20 cells/13 rats; L5: 15 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. H ’ IPSC charge-transfer for adult rat and humans (L2/3: 22 cells/13 cases; L5: 8 cells/5 cases). Data shown as boxplots (25–75% range) with median indicated and whiskers indicating maxima/minima, showing individual points. Statistics from LMM with 2-sided Tukey post-hoc tests: * P < 0.05, ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file.

Article Snippet: Sections were incubated in primary antibodies raised against GABA B1 (mouse, 1:400, N93A/49, NeuroMab, UNC Davis, CA, USA) and NeuN (rabbit, 1:500, ABN78, Millipore) diluted in PBS containing 5% NGS, 0.3% TritonX-100 and 0.05% NaN 3 , for 24–72 h at 4 °C.

Techniques: Immunofluorescence, Control

A Responses of human L2/3 PCs to depolarising current (500 pA, 500 ms), from seizure-free patients (black), patients with seizures and received LEV (red, SZ&LEV), or patients not experiencing seizures, but receiving LEV (purple). B Current – frequency responses (0–500 pA, 500 ms), from control (24 cells/15 cases), SZ&LEV (24 cells/11 cases), and LEV (13 cells/4 cases). C GABA B R IPSCs evoked in L2/3 PCs from control (22 cells/13 cases), SZ&LEV (19 cells/10 cases), and LEV (8 cells/4 cases), superimposed response in CGP (light traces) and IPSC amplitudes for each patient group. D Whole-cell current following baclofen and CGP wash-in for control (27 cells/17 cases), SZ&LEV (18 cells/11 cases), and LEV (6 cells/4 cases) patients. E Baclofen current-density for control (34 cells/18 cases), SZ&LEV (23 cells/13 cases), and LEV (7 cells/4 cases) patients (Ctrl vs. SZ&LEV: p = 0.014; Ctrl vs. LEV-alone: p = 0.048; SZ&LEV vs. LEV-alone: p = 0.705; Tukey post hoc tests). F Baclofen current-density in slices pre-treated for 3 h with 100 μM LEV or vehicle, from control (Veh: 6 cells/4 cases; LEV: 8 cells/5 cases) and SZ&LEV patients (Veh: 7 cells/5 cases; LEV: 7 cells/4 cases). G Power spectra from L2/3 LFP recordings in KA+CCh from control (8 slices/8 cases), SZ&LEV (6 slices/6 cases), and LEV (4 slices/4 cases) patients. H Relative change in AUC from LEV-only patients compared to control following 2 μM (left) or 20 μM (right) baclofen wash-in. All data is shown as either mean ± SEM ( B , D , G ) or boxplots (25−75% range) with the median indicated and whiskers indicating maximum/minimum ranges ( C , E , F , H ) with individual values overlain. Statistics from LMM ( C ), 1-way ANOVA ( E ) with Holm–Sidak tests, 2-way (2-sided, B , F ), or 3-way ANOVA ( H ).

Journal: Nature Communications

Article Title: Phylogenetic divergence of GABA B receptor signaling in neocortical networks over adult life

doi: 10.1038/s41467-025-59262-8

Figure Lengend Snippet: A Responses of human L2/3 PCs to depolarising current (500 pA, 500 ms), from seizure-free patients (black), patients with seizures and received LEV (red, SZ&LEV), or patients not experiencing seizures, but receiving LEV (purple). B Current – frequency responses (0–500 pA, 500 ms), from control (24 cells/15 cases), SZ&LEV (24 cells/11 cases), and LEV (13 cells/4 cases). C GABA B R IPSCs evoked in L2/3 PCs from control (22 cells/13 cases), SZ&LEV (19 cells/10 cases), and LEV (8 cells/4 cases), superimposed response in CGP (light traces) and IPSC amplitudes for each patient group. D Whole-cell current following baclofen and CGP wash-in for control (27 cells/17 cases), SZ&LEV (18 cells/11 cases), and LEV (6 cells/4 cases) patients. E Baclofen current-density for control (34 cells/18 cases), SZ&LEV (23 cells/13 cases), and LEV (7 cells/4 cases) patients (Ctrl vs. SZ&LEV: p = 0.014; Ctrl vs. LEV-alone: p = 0.048; SZ&LEV vs. LEV-alone: p = 0.705; Tukey post hoc tests). F Baclofen current-density in slices pre-treated for 3 h with 100 μM LEV or vehicle, from control (Veh: 6 cells/4 cases; LEV: 8 cells/5 cases) and SZ&LEV patients (Veh: 7 cells/5 cases; LEV: 7 cells/4 cases). G Power spectra from L2/3 LFP recordings in KA+CCh from control (8 slices/8 cases), SZ&LEV (6 slices/6 cases), and LEV (4 slices/4 cases) patients. H Relative change in AUC from LEV-only patients compared to control following 2 μM (left) or 20 μM (right) baclofen wash-in. All data is shown as either mean ± SEM ( B , D , G ) or boxplots (25−75% range) with the median indicated and whiskers indicating maximum/minimum ranges ( C , E , F , H ) with individual values overlain. Statistics from LMM ( C ), 1-way ANOVA ( E ) with Holm–Sidak tests, 2-way (2-sided, B , F ), or 3-way ANOVA ( H ).

Article Snippet: Sections were incubated in primary antibodies raised against GABA B1 (mouse, 1:400, N93A/49, NeuroMab, UNC Davis, CA, USA) and NeuN (rabbit, 1:500, ABN78, Millipore) diluted in PBS containing 5% NGS, 0.3% TritonX-100 and 0.05% NaN 3 , for 24–72 h at 4 °C.

Techniques: Control